Question

Polymorphism in RAPD is observed because ................

• A. DNA used is from different chromosomes of same species
• B. DNA used is from same chromosomes of same species
• C. DNA used is from different chromosomes of different species
• D. DNA used is from complementary chromosomes of same species

Hint:

RAPD is a valuable tool for detecting genetic variation, and polymorphisms are observed when DNA from different chromosomes or species is used.

Answer 1

The Correct Option is C

Random Amplified Polymorphic DNA (RAPD) is a technique used to detect genetic variation by amplifying random segments of DNA. Polymorphisms in RAPD arise because of genetic differences between individuals or species. The presence of different genetic sequences results in distinct patterns of DNA amplification.

(1) RAPD Polymorphism:
- RAPD analysis relies on the fact that different DNA sequences can cause different patterns of amplification when primers are used.
- The observed polymorphisms occur when DNA from different chromosomes or species varies, leading to different amplified regions.

(2) Why Other Options Are Incorrect:
- Option 1: DNA from the same species but from different chromosomes can still lead to polymorphisms, but it doesn't fully explain the variation in RAPD.
- Option 2: DNA from the same chromosomes of the same species would likely not show polymorphisms since there would be little genetic variation.
- Option 4: Complementary chromosomes are not a relevant factor in RAPD polymorphisms.

Conclusion: Polymorphism in RAPD occurs due to variations in DNA from different chromosomes of different species, resulting in different amplified patterns.