Question

PCR is used for

• A. DNA amplification
• B. DNA isolation
• C. DNA ligation
• D. DNA digestion

Answer 1

The Correct Option is A

• PCR (Polymerase Chain Reaction) is a technique primarily used for DNA amplification. It allows the production of many copies of a specific DNA segment.
• The process involves repeated cycles of heating and cooling to denature the DNA, anneal primers, and extend the DNA strands, thus amplifying the target sequence.

The correct answer is (A) : DNA amplification.

Answer 2

The correct answer is: (A) DNA amplification.

Polymerase Chain Reaction (PCR) is a widely used laboratory technique in molecular biology that allows the amplification of a specific segment of DNA. It was developed by Kary Mullis in 1983 and has since revolutionized genetic research, diagnostics, and forensic science.

In PCR, a small amount of DNA is used as a template to create millions of copies of a specific DNA sequence. This is achieved through a series of temperature-controlled cycles, involving denaturation (separating the DNA strands), annealing (binding primers to the DNA template), and extension (synthesizing new DNA strands). The result is a large quantity of the target DNA, which can be analyzed or used in further applications.

Applications of PCR include DNA fingerprinting, genetic testing, cloning, diagnosing diseases, and studying genetic mutations. It is an essential tool in modern biotechnology and molecular biology.