$pBR _{322}$, which is frequently used as a vector for cloning gene in $E$. coli is a/an
- original bacterial plasmid
- modified bacterial plasmid
- viral genome
- transposon
The Correct Option is B
Solution and Explanation
Top Questions on recombinant technology
Frequency of recombination between gene pairs on same chromosome as a measure of the distance between genes to map their position on chromosome, was used for the first time by
- NEET (UG) - 2023
- Biology
- recombinant technology
- Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
- NEET (UG) - 2021
- Biology
- recombinant technology
- During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
- NEET (UG) - 2021
- Biology
- recombinant technology
- During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
- NEET (UG) - 2021
- Biology
- recombinant technology
- Which of the following is not an application of $PCR$ (Polymerase Chain Reaction)?
- NEET (UG) - 2021
- Biology
- recombinant technology
Questions Asked in AIIMS exam
Given below are two statements, one is labelled as Assertion A and the other is labelled as Reason R
Assertion (A) : 02 is liberated in the non-cyclic photophosphorylation.
Reason (R) : Liberation of oxygen is due to photolysis of water.
In the light of the above statements, choose the correct answer from the options given below
- AIIMS - 2019
- Photosynthesis in higher plants
- Match the following column I with column II.
- AIIMS - 2019
- cloning
Given below are two statements, one is labelled as Assertion A and the other is labelled as Reason R
Assertion (A) : The Cro-Magnon man was the direct ancestor of the living modern man.
Reason (R) : Cro-Magnon man had slightly prognathous face.
In the light of the above statements, choose the correct answer from the options given below
- AIIMS - 2019
- evolution
- Given below are two statements, one is labelled as Assertion A and the other is labelled as Reason R
Assertion: Orbit of a satellite is within the gravitational field of earth whereas escaping is beyond the gravitational field of earth.
Reason: Orbital velocity of a satellite is greater than its escape velocity.
In the light of the above statements, choose the correct answer from the options given below- AIIMS - 2019
- Gravitation
Given below are two statements, one is labelled as Assertion A and the other is labelled as Reason R
Assertion (A) : In eukaryotes, transcription occurs in nucleus.
Reason (R) : In bacteria, transcription and translation occurs in cytoplasm.
In the light of the above statements, choose the correct answer from the options given below
- AIIMS - 2019
- Transcription
Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.