Let's use algebra to solve this problem:
Let "x" be the initial quantity of water in the second test tube.
Initially, the first test tube contains some acid and the second test tube contains "x" grams of water.
After pouring 20 grams of acid into the second test tube, the second test tube contains "x + 20" grams of a solution consisting of both water and acid.
Now, two-thirds of this solution is poured from the second test tube into the first test tube.
So, the first test tube contains \((\frac{2}{3})\)(x + 20) grams of the solution.
According to the problem, the fluid in the first test tube is four times that in the second.
Therefore, we can set up the equation:
\((\frac{2}{3})\)(x + 20) = 4x
Now, let's solve for "x":
Multiply both sides of the equation by 3 to get rid of the fraction:
2(x + 20) = 12x
Distribute on the left side:
2x + 40 = 12x
Subtract 2x from both sides:
40 = 10x
Now, divide by 10:
x = 40
So, the initial quantity of water in the second test tube was 40 grams.
The correct option is (C): 40 grams