Question

Name and arrange the following in the order of technique and steps for separation and isolation of DNA fragments:
[I.] DNA fragments moved towards anode under electric field
[II.] Exposure to UV radiation
[III.] DNA fragments stained with EtBr (Ethidium Bromide)
[IV.] Gel electrophoresis
[V.] Elution

• A. V, II, III, I, IV
• B. IV, V, I, III, II
• C. III, II, I, V, IV
• D. IV, I, III, II, V

Hint:

The order is: Gel electrophoresis → Migration → Staining → UV detection → Elution.

Answer 1

The Correct Option is D

To separate and isolate DNA fragments in molecular biology, the correct procedural steps are:
1. IV. Gel electrophoresis: The technique used to separate DNA based on size.
2. I. DNA fragments move towards the anode: DNA is negatively charged and migrates through the gel when current is applied.
3. III. DNA fragments are stained with Ethidium Bromide (EtBr): This helps in visualizing the DNA bands under UV light.
4. II. Exposure to UV radiation: DNA bands stained with EtBr glow under UV light, allowing identification.
5. V. Elution: The desired DNA fragment is then cut out and extracted (eluted) from the gel.

Answer 2

Name and arrange the following in the order of technique and steps for separation and isolation of DNA fragments:

The process of separating and isolating DNA fragments typically involves several key steps:

IV. Gel electrophoresis - This technique is used to separate DNA fragments based on their size by applying an electric field to a gel matrix.

I. DNA fragments moved towards anode under electric field - During electrophoresis, negatively charged DNA fragments migrate towards the positively charged anode.

III. DNA fragments stained with EtBr (Ethidium Bromide) - After separation, DNA fragments are stained with Ethidium Bromide, a dye that intercalates between DNA bases and fluoresces under UV light.

II. Exposure to UV radiation - The stained gel is exposed to UV radiation to visualize the DNA bands as glowing under UV light.

V. Elution - Finally, the desired DNA fragments are extracted or eluted from the gel for further use.

Correct Order: IV, I, III, II, V