Question

Modified Lowry’s Procedure is used to characterize:

• A. Protein-Content in allergen product
• B. Protein profile in allergen product
• C. Potency of allergen product
• D. Storage condition of allergen product

Answer 1

The Correct Option is A

The question at hand is concerned with identifying what the Modified Lowry's Procedure is used for when characterizing an allergen product. The correct answer is "Protein-Content in allergen product." Let us explain the reasoning behind this.

The Modified Lowry's Procedure is a well-established biochemical assay used for determining the protein concentration in a sample. This method is a modification of the classic Lowry assay, which itself is based on two main reactions:

1. The Biuret reaction, where protein in an alkaline solution reduces copper ions to produce a violet color.
2. A subsequent reaction involving the reduction of Folin-Ciocalteu reagent by the peptide bonds and amino acids in the protein, which also develops a blue color.

The intensity of the color produced is proportional to the protein concentration and can be measured spectrophotometrically, typically at a wavelength of around 750 nm.

Now, let us examine the options:

• Protein-Content in allergen product: This is the correct answer. The Modified Lowry’s Procedure is specifically designed to accurately quantify the amount of protein present in a sample.
• Protein profile in allergen product: This refers to identifying specific proteins or characterizing their types, which is generally done using techniques like SDS-PAGE or mass spectrometry.
• Potency of allergen product: Potency evaluation assesses the biological activity or effect of the allergen product, which is not directly linked to determining protein content.
• Storage condition of allergen product: The method does not deal with assessing storage conditions, which generally involves stability tests.

In conclusion, the Modified Lowry's Procedure is utilized primarily to determine the protein content within an allergen product due to the specificity of the assay in quantifying protein concentration.