Remember to associate enzymes with pathways by their primary regulatory or rate-limiting role. For example, PFK-I is central to glycolysis, while Rubisco is unique to the Calvin cycle.
Step 1: Match the pathways with their characteristic enzymes.
$\beta$-Oxidation: Involves the breakdown of fatty acids; Thiolase catalyzes the cleavage of carbon-carbon bonds — Match: A – IV
Glycolysis: The key regulatory enzyme is Phosphofructo kinase-I (PFK-I) — Match: B – II
Gluconeogenesis: A key enzyme unique to this pathway is Phosphoenolpyruvate carboxylase — Match: C – III
Calvin cycle: In photosynthetic organisms, Ribulose Bisphosphate Carboxylase (Rubisco) is the primary enzyme — Match: D – I Step 2: Final matching
A – IV (Thiolase)
B – II (Phosphofructo kinase-I)
C – III (Phosphoenolpyruvate carboxylase)
D – I (Ribulose Bisphosphate Carboxylase)