In matching questions, look for unique functions or characteristics that clearly identify each item. Keywords like "Ori", "marker", and "vector" give strong clues.
A. Ori — IV. Controlling the copy number of linked DNA
Ori (origin of replication) determines how many copies of the plasmid are produced in a cell.
B. Selectable marker — III. Permitting the growth of transformants
Selectable markers help in identifying cells that have taken up the vector by allowing only those cells to grow.
C. Cloning site — II. Single recognition site to link alien DNA
The cloning site contains restriction enzyme recognition sites to insert foreign DNA.
D. Cloning vector — I. Low molecular weight
Cloning vectors are typically small plasmids that replicate efficiently and are easy to manipulate.