Question:

Make a chart(with diagrammatic representation)showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and product it produces.

CBSE CLASS XII

Updated On: Sep 30, 2023

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Solution and Explanation

The name of the restriction enzyme is Bam H 1.

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Concepts Used:

Recombinant Technology

Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.

Process of Recombinant DNA Technology:

Step 1- Isolation of Genetic Material.

Step 2- Cutting the gene at the recognition sites.

Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)

Step 4- Ligation of DNA Molecules.

Step 5- Insertion of Recombinant DNA into Host.

Application of Recombinant DNA Technology:

In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
Recombinant DNA technology is also used to produce Insulin.
ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.