Question:
Make a chart(with diagrammatic representation)showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and product it produces.
Make a chart(with diagrammatic representation)showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and product it produces.
Updated On: Sep 30, 2023
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Solution and Explanation
The name of the restriction enzyme is Bam H 1.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
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- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
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