Question:

In vitro clonal propagation in plants is characterized by

Updated On: Jul 20, 2024
  • PCR and RAPD
  • northern blotting
  • electrophoresis and HPLC
  • microscopy.
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Solution and Explanation

Clonal propagation represents the technique in which vegetative tissue is used to produce plants that are genetically identical to their parents. It provides rapid vegetative multiplication of plant material for agriculture, horticulture and forestry. It can be characterized by PCR and RAPD. The polymerase chain reaction (PCR) technique, generates microgram (pg) quantities of DNA copies (upto billion copies) of the desired DNA (or RNA) segment, present even as a single copy in the initial preparation, in a matter of few hours. RAPD stands for Random Amplification of Polymorphic DNA. It is a type of PCR, but the segments of DNA that are amplified are random. No knowledge of the DNA sequence for the i targeted gene is required, as the primers will bind somewhere in the sequence, but it is not certain exactly where. Its resolving power is much lower than targeted
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Concepts Used:

Strategies For Enhancement in Food Production

Animal Husbandry

The raising and breeding of livestock are known as animal husbandry. Dairy farm management and fisheries are also included under animal husbandry and responsible for the selection of good breeds for better yield, maintaining a well-ventilated house and proper cleanliness and hygiene, and regulating proper disposal of waste.

Read More: Strategies for Enhancement in Food Production

Animal Breeding

The practice of raising a group of animals belonging to a common species. Inbreeding and outbreeding are the two types of animal breeding.

  1. Animals of superior male and female breed are made to mate for 4 to 6 generations. According to Mendel's experiment, true-breeding species or pure lines are created by Inbreeding which results in homozygosity.
  2. The breeding involving animals that are not related is known as outbreeding.
  3. Bee-keeping: The rearing of bees for the production of honey and beeswax is known as apiculture. One of the most common species of the honey bee is Apis indica. Beeswax is used to prepare polish, cosmetics, etc.
  4. Fisheries: Cultivation or breeding of aquatic flora and fauna for a commercial purpose is known as aquaculture. Fish and fish products for consumption are known as pisciculture.

Plant Breeding

In order to get rid of nutrient deficiency diseases, crops need to have a specific nutrient value besides disease resistance, pest resistance, and high yield capacity. There are many strategies for enhancing food production through plant breeding.

  1. Somatic Hybrids
  2. Totipotency
  3. Biofortification

Plant Breeding for Disease Resistance

Plant Breeding for Pest Resistance

Plant Breeding for Improved Food Quality

Single-Cell Protein

One of the ways to meet the growing demand of nutrients in plants and animals is a single-cell protein which is biomass that is rich in protein. microbes are produced on an industrial scale to get single-cell protein.