Question

In RNA interface, the dsRNA molecule prevents ________

• A. transcription of mRNA
• B. transport of RNA from nucleus to cytoplasm
• C. translation of mRNA
• D. aminoacylation

Answer 1

The Correct Option is C

dsRNA in RNAi:

1. Processed into siRNA by Dicer.
2. siRNA binds to RISC complex, guiding it to cleave complementary mRNA → blocks translation.

Translation of mRNA is the process by which a cell interprets the genetic code in messenger RNA (mRNA) to synthesize a corresponding protein. It occurs in the cytoplasm of the cell and is facilitated by ribosomes, which read the mRNA sequence and assemble the protein by linking amino acids in the correct order. This process is part of the overall gene expression process, following transcription, where DNA is transcribed into mRNA.

Not affected:

• (A) Transcription: Occurs in nucleus.
• (B) RNA transport: Unrelated to dsRNA.
• (D) Aminoacylation: tRNA charging process.

Correct Answer: (C) Translation of mRNA

Answer 2

In RNA interference (RNAi), double-stranded RNA (dsRNA) is processed into small interfering RNAs (siRNAs) by the enzyme Dicer. These siRNAs integrate into the RNA-induced silencing complex (RISC), which then binds to complementary mRNA molecules. 

This binding prevents translation of the target mRNA into protein, effectively silencing gene expression. While dsRNA can theoretically affect other processes, its primary and most well-characterized role in RNAi is to block translation rather than transcription (A), RNA transport (B), or aminoacylation (D).

The correct answer is (C) translation of mRNA.