Step 1: During glycolysis, 2 ATP molecules are consumed in the early steps to convert glucose into a more reactive form. Later in glycolysis, 4 ATP molecules are produced, resulting in a net gain of 2 ATP.
Step 2:
Glycolysis occurs in the cytoplasm and involves the breakdown of glucose into pyruvate, yielding 2 ATP molecules.
Conclusion: The correct answer is that 2 ATP molecules are used in glycolysis.