Question

Explain any two differences between c-DNA and s-DNA.

Hint:

c-DNA is crucial for studying gene expression, while s-DNA is typically used for applications in DNA analysis and hybridization.

Answer 1

c-DNA (complementary DNA) and s-DNA (single-stranded DNA) differ in their structure, function, and formation. Step 1: Definition.
- c-DNA: c-DNA is synthesized from messenger RNA (mRNA) through the process of reverse transcription. It represents the coding sequence of genes. - s-DNA: s-DNA is a single-stranded form of DNA, which can be either a naturally occurring DNA molecule or the result of denaturation.
Step 2: Differences.
1. Formation: - c-DNA is synthesized from mRNA through reverse transcription. - s-DNA can be generated by denaturation or by naturally occurring single-stranded regions. 2. Purpose: - c-DNA is used to study gene expression and to clone genes. - s-DNA is typically used in hybridization experiments and may be involved in gene mapping.
Final Answer: 1. c-DNA is synthesized from mRNA, while s-DNA can be obtained by denaturation. 2. c-DNA represents gene expression, while s-DNA is used in hybridization studies.