Question

Expand PCR. Mention its any one importance in biotechnology.

Hint:

The enzyme {Taq} polymerase is isolated from the bacterium {Thermus aquaticus}, which remains active during the high-temperature denaturation step.

Answer 1

Step 1: PCR stands for Polymerase Chain Reaction. It is a technique used to make multiple copies of a specific DNA sequence in vitro.
Step 2: It involves three main steps: Denaturation, Annealing, and Extension, using a thermostable DNA polymerase called Taq polymerase.
Step 3: Importance: It is crucial for early diagnosis of diseases (like AIDS or cancer), DNA fingerprinting, and gene cloning when only a very small amount of DNA is available.