Question:
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Updated On: Jul 26, 2024
- Annealing
- Extension
- Denaturation
- Ligation
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The Correct Option is C
Solution and Explanation
During $PCR$ process, if high temperature is not maintained, the initial step of the process known as denaturation of DNA is not done.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
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