DNA fingerprinting involves identifying differences in specific regions in DNA sequence.
DNA fingerprinting is the basis of paternity testing.
- If both assertion and reason are true and reason is the correct explanation of assertion
- If both assertion and reason are true but reason is not the correct explanation of assertion
- If assertion is true but reason is false
- If both assertion and reason are false
The Correct Option is B
Solution and Explanation
Top Questions on DNA fingerprinting
- Arrange the given steps of DNA finger printing technique in correct sequence.
A. Isolation of DNA and its digestion by restriction and endonuclease
B. Seperation of DNA fragments by electrophoresis
C. Hybridization with labelled VNTR probe and autoradiography
D. Blotting of seperated DNA fragments to synthetic membrane
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
- The steps of DNA fingerprinting are given below. Arrange them in a correct sequence.
(A)Separation of DNA fragments by electrophoresis
(B)Hybridization using labelled VNTR probe
(C)Isolation and digestion of DNA
(D)Detection of hybridized DNA fragments by autoradiography
(E)Transferring Of separated DNA fragments to synthetic membranes
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
Which aspect forms the basis of DNA finger- printing?
- KCET - 2022
- Biology
- DNA fingerprinting
- DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
- DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as:
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
Questions Asked in AIIMS exam
Given below are two statements, one is labelled as Assertion A and the other is labelled as Reason R
Assertion (A) : 02 is liberated in the non-cyclic photophosphorylation.
Reason (R) : Liberation of oxygen is due to photolysis of water.
In the light of the above statements, choose the correct answer from the options given below
- AIIMS - 2019
- Photosynthesis in higher plants
- Match the following column I with column II.
- AIIMS - 2019
- cloning
Given below are two statements, one is labelled as Assertion A and the other is labelled as Reason R
Assertion (A) : The Cro-Magnon man was the direct ancestor of the living modern man.
Reason (R) : Cro-Magnon man had slightly prognathous face.
In the light of the above statements, choose the correct answer from the options given below
- AIIMS - 2019
- evolution
- Given below are two statements, one is labelled as Assertion A and the other is labelled as Reason R
Assertion: Orbit of a satellite is within the gravitational field of earth whereas escaping is beyond the gravitational field of earth.
Reason: Orbital velocity of a satellite is greater than its escape velocity.
In the light of the above statements, choose the correct answer from the options given below- AIIMS - 2019
- Gravitation
Given below are two statements, one is labelled as Assertion A and the other is labelled as Reason R
Assertion (A) : In eukaryotes, transcription occurs in nucleus.
Reason (R) : In bacteria, transcription and translation occurs in cytoplasm.
In the light of the above statements, choose the correct answer from the options given below
- AIIMS - 2019
- Transcription
Concepts Used:
DNA Fingerprinting
DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, method of isolating and identifying variable elements within the base-pair sequence of DNA.
The procedure of DNA Fingerprinting:
The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis): the shorter the fragment, the more quickly it moved toward the positive pole (anode). The sorted double-stranded DNA fragments were then subjected to a blotting technique in which they were split into single strands and transferred to a nylon sheet. The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached. The resultant pattern of marks could then be analyzed.