Question

DNA denaturation is an example of which type of shift?

• A. Bathochromic
• B. Blue shift
• C. Hyperchromic shift
• D. Red shift

Answer 1

The Correct Option is C

Step 1: Understanding DNA denaturation. - DNA denaturation refers to the disruption of hydrogen bonds between base pairs, leading to strand separation. - This process results in an increase in UV absorbance due to unstacking of bases. 

Step 2: Explanation of incorrect options. - (A) Bathochromic shift: Refers to a shift to longer wavelengths (red shift) but is not related to DNA denaturation. - (B) Blue shift: Refers to a shift to shorter wavelengths, which does not occur in DNA denaturation. - (D) Red shift: Similar to a bathochromic shift but does not describe the absorbance increase seen in denaturation. 

Step 3: Selecting the correct option. Since DNA denaturation leads to an increase in UV absorbance, the correct term is hyperchromic shift. Hence, the correct answer is (C) Hyperchromic shift.