Question

Correctly match the enzymes used as selectable markers (Group I) and the chemicals used for their selection (Group II):

• A. P-2, Q-1, R-3, S-4
• B. P-1, Q-2, R-3, S-4
• C. P-2, Q-4, R-1, S-3
• D. P-3, Q-4, R-1, S-2

Hint:

When selecting transformants in plant biotechnology, enzymes like Neomycin phosphotransferase and Phosphinothricin acetyltransferase are used in conjunction with chemicals like Kanamycin and Bialaphos to identify successful transformations.

Answer 1

The Correct Option is A

Let’s break down the matching between the enzymes and the chemicals: 
- (P) Neomycin phosphotransferase: This enzyme confers resistance to the chemical Kanamycin. Hence, the correct match for Neomycin phosphotransferase is \( P-2 \). 
- (Q) Phosphinothricin acetyltransferase: This enzyme provides resistance to Bialaphos, a herbicide. Hence, the correct match for Phosphinothricin acetyltransferase is \( Q-1 \). 
- (R) Dihydrofolate reductase: This enzyme provides resistance to Methotrexate, a chemotherapy agent, and is commonly used in plant transformations for selection. Hence, the correct match for Dihydrofolate reductase is \( R-3 \). 
- (S) 5-Enolpyruvyl shikimate 3-phosphate synthase: This enzyme provides resistance to Glyphosate, a widely used herbicide. Hence, the correct match for 5-Enolpyruvyl shikimate 3-phosphate synthase is \( S-4 \). 
Thus, the correct answer is (A), with the matching being \( P-2 \), \( Q-1 \), \( R-3 \), and \( S-4 \).