Question

Choose the correct statements regarding plant tissue culture -
A. Organogenesis is inducing the formation of various vegetative organs from cells or tissues.
B. Formation of mass of undifferentiation cells from callus redifferentiation.
C. Cytoplasmic hybrids are prepared by taking the nucleus from one parent and cytoplasm from both the parents.
D. Relative concentration of growth hormones play important role in organogenesis.
Choose the correct answer from the options given below :

• A. A, B, and C Only
• B. B, C and D Only
• C. A and B Only
• D. A, C and D Only

Hint:

Remember the auxin-to-cytokinin ratio rule: High Auxin/Cytokinin ratio generally induces root formation, while a Low Auxin/Cytokinin ratio induces shoot formation.

Answer 1

The Correct Option is D

Step 1: Understanding the Concept:
The question requires evaluating the correctness of four statements related to different aspects of plant tissue culture.
Step 2: Detailed Explanation:
A. Organogenesis is inducing the formation of various vegetative organs from cells or tissues. This is the correct definition of organogenesis in the context of plant tissue culture. (Correct)
B. Formation of mass of undifferentiation cells from callus redifferentiation. This statement is incorrect. The formation of an undifferentiated cell mass (callus) from a differentiated explant is called dedifferentiation. The formation of organs from the callus is called redifferentiation. The statement has these terms reversed. (Incorrect)
C. Cytoplasmic hybrids are prepared by taking the nucleus from one parent and cytoplasm from both the parents. This describes the creation of a cybrid (cytoplasmic hybrid). This is typically achieved by fusing a normal protoplast (nucleus + cytoplasm) of one parent with an enucleated protoplast (cytoplasm only) of the other, resulting in a cell with the nucleus from one parent and a mixed cytoplasm from both parents. (Correct)
D. Relative concentration of growth hormones play important role in organogenesis. This is a fundamental principle of plant tissue culture. The ratio of auxins to cytokinins in the culture medium determines whether the callus will differentiate into roots, shoots, or remain undifferentiated. (Correct)
Step 3: Final Answer:
Statements A, C, and D are correct. Therefore, the correct option is A, C and D Only.