Step 1: Understanding the Concept:
The question is about the production and characteristics of "doubled haploids," which are homozygous diploid plants used in plant breeding.
Step 2: Detailed Explanation:
A. They are produced by doubling the chromosome number of haploids. This is the definition of a doubled haploid. Haploid plants (n) are first produced via anther or ovule culture, and then their chromosome set is doubled to create a completely homozygous diploid (2n) plant. (Correct)
B. Doubling of chromosome enables the recessive traits to express too. In a haploid organism, every gene (including recessive alleles) is expressed because there is no other allele to mask it. When the chromosomes are doubled (e.g., a haploid with allele 'r' becomes a diploid 'rr'), the recessive trait remains expressed and is now fixed in a homozygous state. (Correct)
C. Chromosome doubling is done by Ozone treatment. Ozone is a strong oxidizing agent used for sterilization, not for inducing polyploidy. This statement is (Incorrect).
D. Chromosome doubling is done by colchicine treatment. Colchicine is a chemical extracted from the autumn crocus. It is a mitotic inhibitor that disrupts the formation of the spindle fibers during cell division. This prevents the separation of chromosomes, leading to a doubling of the chromosome number in the cell. This is the standard method used for producing doubled haploids. (Correct)
Step 3: Final Answer:
Statements A, B, and D are correct.