Question

Choose the correct statements among the following
I. Pure DNA can be isolated by using ribonuclease and proteases.
II. Ethidium bromide staining facilitates the visualisation of DNA fragment in Agarose gel.
III. Sticky and ligation techniques help to cut the gene of interest from the source DNA.

• A. I, III
• B. II only
• C. I, II, III
• D. I only

Hint:

When asked to identify correct statements, evaluate each one independently and fact-check using core scientific principles.

Answer 1

The Correct Option is C

Statement I: Ribonuclease and proteases remove RNA and proteins respectively, allowing pure DNA isolation.
Statement II: Ethidium bromide binds to DNA and fluoresces under UV light, making DNA visible in gel electrophoresis.
Statement III: Restriction enzymes create sticky ends, and DNA ligase joins the desired DNA fragments — enabling gene extraction. All three statements are correct.