Question

Cell suspension cultures are grown in

• A. Semisolid media
• B. Liquid media
• C. Solid media
• D. Gaseous media

Hint:

• Liquid media Ideal for suspension cultures due to free movement of cells.
• Solid/Semisolid media Used for adherent or callus cultures.
• Gaseous media Not applicable for cell suspension cultures.

Answer 1

The Correct Option is B

Cell suspension cultures are a type of culture technique used in biotechnology to grow plant cells, tissues, or small plant organs in a medium that provides the necessary nutrients. This technique is important for processes such as the production of secondary metabolites, genetic modifications, and plant research.

Cell suspension cultures are typically grown in liquid media. The liquid medium allows the cells to be evenly distributed and provides the cells with an environment where nutrients and gases can be easily accessed. Liquid media are preferable for suspension cultures because:

• They provide a consistent environment for cell growth and division.
• The constant motion in bioreactors or shakers ensures that cells receive homogeneous nutrient distribution.
• It allows for easier harvesting of cells and products.
• Gaseous exchange is facilitated, promoting better cell metabolism.

Therefore, the correct answer to the question is Liquid media.