Beer Lambert's law gives the relation between which of the following?
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Beer-Lambert Law. Relates light absorbance to concentration and path length: \(A = \epsilon c l\). Absorbance \(A\) is directly related to the amount of light energy absorbed by the sample.
The Beer-Lambert Law (or Beer's Law) is fundamental to absorption spectroscopy (like UV-Vis spectrophotometry) It relates the attenuation (decrease in intensity) of light as it passes through a substance to the properties of that substance Specifically, it states that the absorbance (A) of a solution is directly proportional to the concentration (c) of the absorbing species and the path length (l) the light travels through the solution
$$ A = \epsilon c l $$
where \(\epsilon\) is the molar absorptivity (a constant for a given substance at a specific wavelength) Absorbance is defined as \( A = \log_{10}(I_0 / I) \), where \(I_0\) is the incident light intensity and \(I\) is the transmitted light intensity The difference (\(I_0 - I\)) represents the light energy absorbed (or scattered/reflected, but the law primarily focuses on absorption) Therefore, the law fundamentally relates the absorption of light energy to the concentration of the absorbing substance