Question

Arrange the given steps involved in polymerase chain reaction (PCR):
(A) Denaturation
(B) Annealing
(C) Extension
(D) Cycling
Choose the correct answer from the options given below:

• A. (A), (B), (C), (D)
• B. (A), (C), (B), (D)
• C. (B), (A), (D), (C)
• D. (C), (B), (D), (A)

Hint:

Understand the sequence of steps in PCR and their roles in DNA amplification.

Answer 1

The Correct Option is A

The steps of PCR are as follows:
1. Denaturation: Heat the double-stranded DNA to separate it into single strands.
2. Annealing: Lower the temperature to allow primers to bind to complementary sequences.
3. Extension: DNA polymerase extends the primers by synthesizing a new strand of DNA.
4. Cycling: These steps are repeated multiple times to amplify the target DNA sequence.