The steps of PCR are as follows:
1. Denaturation: Heat the double-stranded DNA to separate it into single strands.
2. Annealing: Lower the temperature to allow primers to bind to complementary sequences.
3. Extension: DNA polymerase extends the primers by synthesizing a new strand of DNA.
4. Cycling: These steps are repeated multiple times to amplify the target DNA sequence.
List I | List II |
---|---|
(A) Western Blotting | (II) Protein identification |
(B) Southern Blotting | (I) DNA identification |
(C) Northern Blotting | (IV) RNA identification |
(D) Yeast two-hybrid system | (III) Protein-protein interaction |