Question:
Isoelectric focusing allows separation of the proteins according to their:
Isoelectric focusing allows separation of the proteins according to their:
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Understand the concept of isoelectric point (pI) and its role in protein separation techniques.
Updated On: Feb 27, 2025
- pH
- pI
- pKa
- pKb
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The Correct Option is B
Solution and Explanation
Isoelectric focusing (IEF) separates proteins based on their isoelectric point (pI), which is the pH at which the protein has no net charge. Proteins migrate in a pH gradient during IEF until they reach the pH corresponding to their pI, where they cease to move as the net charge becomes zero.
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