Question

Arrange the following steps for plant tissue culture (P.T.C.) in correct sequence:
A. Selection of desired material and suitable nutrient media for P. T. C.
B. Inoculation of explants
C. Surface sterilization of explant
D. Transfer of growing cultures
E. Transfer of plantlets to soil in pots
Choose the correct answer from the options given below :

• A. A, B, C, D, E
• B. A, C, B, D, E
• C. A, C, E, B, D
• D. B, C, A, E, D

Hint:

Remember the logic of asepsis in biology labs: {Prepare} (A), {Sterilize} (C), then {Inoculate} (B). Never inoculate before sterilizing the explant. The final step is always moving the plantlet out of the lab and into the soil (E).

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
The question asks for the correct chronological order of the general steps involved in performing a plant tissue culture experiment, from initiation to the final establishment of plants.
Step 2: Detailed Explanation:
Let's analyze the steps to establish a logical workflow:
A. Selection of desired material and suitable nutrient media for P. T. C.: This is the planning stage and the first step. You must decide which plant and which part of the plant (explant) to use and prepare the appropriate sterile nutrient medium.
C. Surface sterilization of explant: Before introducing the explant into the sterile culture environment, all surface microorganisms must be killed to prevent contamination. This is a critical step.
B. Inoculation of explants: After sterilization, the explant is placed onto or into the sterile nutrient medium inside a culture vessel. This process is called inoculation.
D. Transfer of growing cultures: As the cultures grow, they deplete the nutrients in the medium. They need to be periodically transferred (subcultured) to fresh medium to maintain growth. This step occurs during the in vitro growth phase.
E. Transfer of plantlets to soil in pots: Once the in vitro culture has developed into a complete plantlet with roots and shoots, it must be carefully moved from the sterile, high-humidity lab environment to the non-sterile soil environment. This process is called hardening or acclimatization and is the final stage.
Therefore, the correct sequence is A \(\rightarrow\) C \(\rightarrow\) B \(\rightarrow\) D \(\rightarrow\) E.
Step 3: Final Answer:
The correct sequence of steps for plant tissue culture is A, C, B, D, E.