Question

Arrange the basic steps to develop G. M. O. (Genetically modified organisms) in sequence:
A. Transfer of DNA with desired genes to its progeny
B. Identification of DNA with desired genes
C. Introduction of the DNA into the host.
D. Maintenance of introduced DNA in the host.
Choose the correct answer from the options given below :

• A. A, B, C, D
• B. B, C, A, D
• C. B, A, D, C
• D. B, C, D, A

Hint:

Think of the process logically: {Find} the gene \(\rightarrow\) {Insert} the gene \(\rightarrow\) {Stabilize} the gene in the parent \(\rightarrow\) {Inherit} the gene in the offspring.

Answer 1

The Correct Option is D

Step 1: Understanding the Concept:
The question asks for the logical sequence of steps involved in the creation and establishment of a genetically modified organism.
Step 2: Detailed Explanation:
Let's arrange the steps in a logical order:
B. Identification of DNA with desired genes: The very first step is to identify and isolate the gene of interest that confers the desired trait.
C. Introduction of the DNA into the host: Once the gene is identified and isolated (often cloned into a vector), it must be introduced into the cells of the host organism. This is the transformation step.
D. Maintenance of introduced DNA in the host: After transformation, it's crucial to select the cells that have successfully incorporated the new DNA and ensure that this DNA is stable, either by integrating into the host's genome or by replicating as an episome. The host must be grown and regenerated while maintaining the new gene.
A. Transfer of DNA with desired genes to its progeny: The final goal of creating a GMO, especially in agriculture, is to have a stable line where the new trait is heritable. This means the introduced DNA must be passed on to the next generation through normal reproduction.
The correct sequence is B \(\rightarrow\) C \(\rightarrow\) D \(\rightarrow\) A.
Step 3: Final Answer:
The correct sequence of steps is represented by the option B, C, D, A.