Let's identify the essential components of a UV-Visible Spectrophotometer.
Step 1: Analyze the required components. A UV-Vis spectrophotometer needs:
A light source that covers the UV and visible range.
A device to select a specific wavelength of light.
A sample holder.
A detector.
Step 2: Evaluate the options.
A. Interferometer: This is the key component of an FTIR (Fourier Transform Infrared) spectrometer, not a UV-Vis instrument.
B. Monochromator: This device (using a prism or diffraction grating) is used to select a narrow band of wavelengths from the source light. This is an essential part of a UV-Vis spectrophotometer.
C. Hollow cathode lamp: This is a specific type of lamp used as a light source in Atomic Absorption Spectroscopy (AAS), not UV-Vis.
D. Deuterium lamp: This is the standard light source used to provide radiation in the ultraviolet (UV) range (typically 190-400 nm) in a UV-Vis spectrophotometer. (A tungsten lamp is used for the visible range).
Conclusion: The Monochromator (B) and the Deuterium lamp (D) are both essential components of a UV-Vis spectrophotometer.