Question

Agrobacterium tumefaciens is frequently used as a vector to create transgenic plants. Under laboratory conditions Agrobacterium - mediated plant transformation does not require

• A. Host plant genes
• B. Bacterial type IV secretion system
• C. vir genes
• D. Opine catabolism genes

Hint:

Distinguish between the machinery for DNA transfer and the bacterium's reward. The vir genes and the Type IV secretion system are the machinery. Opine production (by the plant) and catabolism (by the bacterium) are the reward. For lab work, we only need the machinery, not the reward system.

Answer 1

The Correct Option is D

Step 1: Understanding the Concept:
Agrobacterium tumefaciens is a natural genetic engineer. It transfers a segment of its DNA, called T-DNA (transfer DNA), from its Ti (tumor-inducing) plasmid into the genome of a host plant. In biotechnology, this system is harnessed to introduce genes of interest into plants. The question asks which component of the natural system is not necessary for this laboratory application.
Step 2: Detailed Explanation:
Let's review the roles of the listed components in the transformation process:
1. Host plant genes: The process is initiated when the bacterium senses chemical signals (like acetosyringone) released by wounded plant cells. These signals are products of host plant genes. Therefore, host plant gene expression is required to start the process.
2. Bacterial type IV secretion system (T4SS): This is the molecular machinery, like a syringe, that the bacterium assembles to inject the T-DNA complex across its membranes and into the plant cell. It is absolutely essential for the DNA transfer.
3. vir genes: The "virulence" (vir) genes are located on the Ti plasmid and encode the proteins needed for the transformation process. This includes proteins that sense the plant signals, proteins that process the T-DNA, and the proteins that make up the Type IV secretion system. The vir genes are essential.
4. Opine catabolism genes: In the natural system, the T-DNA that is transferred carries genes that force the plant cell to produce unique molecules called opines. The Ti plasmid also carries opine catabolism genes (outside the T-DNA) that allow the bacterium to use these opines as an exclusive food source. While this is the evolutionary advantage for the bacterium in nature, it is irrelevant for the goal of laboratory transformation. In the lab, scientists replace the opine synthesis genes on the T-DNA with their gene of interest. Since no opines are produced, the bacterial genes for breaking them down (catabolism) are not required for the successful transfer of the desired gene.
Step 3: Final Answer:
The opine catabolism genes are only for the benefit of the bacterium after a successful natural infection and are not required for the mechanism of T-DNA transfer in a laboratory setting.