Question

Action of restriction enzyme leads to the formation of sticky ends in both vector DNA and Foreign DNA because of
I. Recognise palindromic nucleotide sequence.
II. Cut the strand of DNA a little away from the centre of palindrome site.
III. Help to form complementary cut counter parts.

• A. I only
• B. II only
• C. I and II
• D. I, II, III

Hint:

Sticky ends = overhangs created by restriction enzymes for ligation efficiency.

Answer 1

The Correct Option is D

All three statements are correct. Restriction enzymes recognize specific palindromic sequences, cut a little off-center and generate sticky ends that are complementary.