The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer)
Which of the following is/are CORRECT for the two molecules shown?
The current passing through the battery in the given circuit, is:
A bob of heavy mass \(m\) is suspended by a light string of length \(l\). The bob is given a horizontal velocity \(v_0\) as shown in figure. If the string gets slack at some point P making an angle \( \theta \) from the horizontal, the ratio of the speed \(v\) of the bob at point P to its initial speed \(v_0\) is :
A full wave rectifier circuit with diodes (\(D_1\)) and (\(D_2\)) is shown in the figure. If input supply voltage \(V_{in} = 220 \sin(100 \pi t)\) volt, then at \(t = 15\) msec:
The process by which the genome’s DNA is copied in cells is called DNA Replication. It must first copy (or replicate) its entire genome before cell division so that each resulting daughter cell ends up with its own complete genome.
It is the natural cycle of creating two indistinguishable imitations of DNA from one unique DNA molecule. DNA replication happens to take all things together living creatures going about as the most core part for organic legacy. This is important for cell division during the development and fixation of harmed tissues, while it likewise guarantees that every one of the new cells gets its duplicate of the DNA. The phone has the unmistakable property of division, which makes replication of DNA fundamental.
The parental DNA stays together, and the recently shaped daughter strands are together in conservative replication. The semi-conservative strategy recommends that every one of the two parental DNA strands goes about as a format for new DNA to be integrated; after that each two-fold abandoned DNA incorporates one "old" strand (parental) and one "new" strand.