Question:
A mixture of substances A, B, C, D is subjected to column chromatography. The degree of adsorption is in the order of \(D>B>C>A\). The column is eluted with a suitable solvent. Identify the correct statement with respect to the separation of the mixture.
A mixture of substances A, B, C, D is subjected to column chromatography. The degree of adsorption is in the order of \(D>B>C>A\). The column is eluted with a suitable solvent. Identify the correct statement with respect to the separation of the mixture.
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In column chromatography:
- The substance with the {lowest adsorption} elutes first.
- The substance with the {highest adsorption} elutes last.
- Adsorption order determines the sequence of separation.
Updated On: Mar 22, 2025
- D comes out first from the column
- A comes out first from the column
- C comes out after B from the column
- B comes out after D from the column
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The Correct Option is B
Solution and Explanation
Explanation:
- In column chromatography, the separation of substances is based on their adsorption affinities.
- The substance with the least adsorption tendency moves the fastest and elutes first, while the substance with the highest adsorption remains in the column the longest.
- Given the adsorption order \(D>B>C>A\), substance A has the least adsorption and, therefore, will elute first.
- The substance with the least adsorption tendency moves the fastest and elutes first, while the substance with the highest adsorption remains in the column the longest.
- Given the adsorption order \(D>B>C>A\), substance A has the least adsorption and, therefore, will elute first.
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