Question:
(a) Draw a schematic diagram of the cloning vector pBR322 and label (1) Bam HI site (2) gene for ampicillin resistance (3) ‘ori’ (4) ‘rop’ gene.
(a) Draw a schematic diagram of the cloning vector pBR322 and label (1) Bam HI site (2) gene for ampicillin resistance (3) ‘ori’ (4) ‘rop’ gene.
Show Hint
The pBR322 vector is one of the most widely used plasmids in genetic engineering due to its multiple cloning sites and selectable markers.
Updated On: Jan 29, 2025
Hide Solution
Verified By Collegedunia
Solution and Explanation
- Bam HI site: A restriction site for cloning.
- Ampicillin resistance gene: A selectable marker.
- ‘Ori’: Origin of replication, essential for plasmid replication.
- ‘Rop’ gene: Regulates plasmid copy number.
Was this answer helpful?
0
0
Top Questions on Ecosystem
- (A) Analyse the following ecosystems and discuss, which will be more productive in terms of primary productivity:
A young forest, a natural old forest, a shallow polluted lake. - Differentiate between Net primary productivity and Gross primary productivity in an ecosystem.
- The process of mineralization by microorganisms helps in the release of:
- Why are restrictions imposed on MTP in India? Up to how many weeks or trimesters, is MTP considered relatively safe for a female, if necessary to perform, by a medical practitioner?
Read the passage given below and answer the question.
Food chains ‘P’ and ‘Q’ form an interconnection. ‘P’ initiates with wheat grass whose population supports a few grasshoppers, frogs and snakes.
‘Q’ starts with dead decaying leaves of wheat grass which are eaten by earthworms and then food chain is continued with frog of food chain ‘P’.
Identify the types of food chains ‘P’ and ‘Q’:
View More Questions
Questions Asked in CBSE CLASS XII exam
- Explain the following observations using Einstein’s photoelectric equation:
(a) Photoelectric emission does not occur from a surface when the frequency of the light incident on it is less than a certain minimum value.
(b) It is the frequency, and not the intensity of the incident light which affects the maximum kinetic energy of the photoelectrons.
(c) The cut-off voltage \( V_0 \) versus frequency \( \nu \) of the incident light curve is a straight line with a slope \( \frac{h}{e} \).
- CBSE CLASS XII - 2025
- Photoelectric Effect
- In the figure, curved lines represent equipotential surfaces. A charge \( Q \) is moved along different paths A, B, C, and D. The work done on the charge will be maximum along the path:
- CBSE CLASS XII - 2025
- Electrostatics
- Three batteries E1, E2, and E3 of emfs and internal resistances (4 V, 2 \(\Omega\)), (2 V, 4 \(\Omega\)) and (6 V, 2 \(\Omega\)) respectively are connected as shown in the figure. Find the values of the currents passing through batteries E1, E2, and E3.
- CBSE CLASS XII - 2025
- Kirchhoff's Laws
- (a)(i) "To promote the green revolution, the Indian government provided fertilizer subsidies to the farmers." Briefly explain any two arguments in favour of such subsidies.
- CBSE CLASS XII - 2025
- Indian Economy
- How did Franz plan to turn the commotion in the classroom to his favour?
- CBSE CLASS XII - 2025
- Literature
View More Questions