Question:
(a) Draw a schematic diagram of the cloning vector pBR322 and label (1) Bam HI site (2) gene for ampicillin resistance (3) ‘ori’ (4) ‘rop’ gene.
(a) Draw a schematic diagram of the cloning vector pBR322 and label (1) Bam HI site (2) gene for ampicillin resistance (3) ‘ori’ (4) ‘rop’ gene.
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The pBR322 vector is one of the most widely used plasmids in genetic engineering due to its multiple cloning sites and selectable markers.
Updated On: Jan 29, 2025
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Solution and Explanation
- Bam HI site: A restriction site for cloning.
- Ampicillin resistance gene: A selectable marker.
- ‘Ori’: Origin of replication, essential for plasmid replication.
- ‘Rop’ gene: Regulates plasmid copy number.
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