List of top Biotechnology Tools and Techniques Questions

Among individuals in a human population, minor variations exist in nucleotide sequences of chromosomes. These variations can lead to gain or loss of sites for specific restriction enzymes. Which of the following techniques is used to identify such variations?
The recognition sequences of four Type-II restriction enzymes (RE) are given below. The symbol (|) indicates the cleavage site. Identify the RE that generates sticky ends.
Which of the following is NOT used for generating an optimal alignment of two nucleotide sequences?
A DNA solution of 50 \(\mu\)g mL\(^{-1}\) gives absorbance of 1.0 at 260 nm. A 20 \(\mu\)L aliquot from a 50 \(\mu\)L purified plasmid solution is diluted to 1000 \(\mu\)L. The diluted solution gives absorbance 0.550 at 260 nm. The purified plasmid concentration in \(\mu\)g \(\mu\)L\(^{-1}\) (rounded to two decimals) is \(\underline{\hspace{1cm}}\).
The possible number of SalI restriction sites in a 9 kb double-stranded DNA with equal base proportion (nearest integer) is \(\underline{\hspace{1cm}}\).
Tertiary structure of a protein consisting of $\alpha$-helices and $\beta$-strands can be determined by:
Which of the following nucleus/nuclei is/are NMR active?
Which one of the following tools is used to compare all the possible six-open reading frames of a given nucleotide query sequence with all the available six-open reading frames of the nucleotide sequence database?
The enzyme which adds phosphate group to the free 5' terminus of a DNA sequence is
Which one of the following techniques/tools is NOT used for inserting a foreign gene into a cell?
CRISPR-Cas system is associated with:
Number of unrooted trees in a phylogeny of five sequences is
Which one of the following methods is used to test the significance of a predicted phylogeny?