Step 1: Historical background.
The concept of the "transforming principle" was first proposed by Frederick Griffith in 1928 during his experiments on \textit{Streptococcus pneumoniae}. He demonstrated that genetic material from heat-killed virulent bacteria could transform non-virulent bacteria into virulent ones. Step 2: Role of other scientists.
Oswald Avery: Later identified the transforming principle as DNA in 1944 through experiments with Avery, MacLeod, and McCarty.
James Watson: Contributed to the discovery of the structure of DNA alongside Francis Crick.
Rosalind Franklin: Provided critical X-ray diffraction data that aided in understanding the structure of DNA.
Step 3: Conclusion.
Frederick Griffith was the first to propose and demonstrate the existence of a transforming principle, though its identity as DNA was confirmed later by Avery and his colleagues. The correct answer is: Frederick Griffith.
Answer the following questions:
(a) [(i)] Explain how some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans but do not kill the Bacillus.
[(ii)] How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists?
(b) [(i)] Explain how the amplification of gene of interest is done using PCR.
[(ii)] State two applications of the desired amplified fragment of DNA.
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a) What is the technical term used for Step 4 in the above process?
(b) Which of the given two combinations of restriction enzyme should be used in Step 1? Justify your answer.
(i) EcoR I to cut the plasmid and Hind III to cut the alien DNA.
(ii) EcoR I to cut both the plasmid and alien DNA.