Question

The cloning site present in the \( \textit{rop}\) site of \(\textit{E. coli}\) cloning vector pBR322 is:

• A. \(( \text{Pvu II} )\)
• B. \(( \text{Pvu II} )\)
• C. \(( \text{Pvu II} )\)
• D. \(( \text{BamH I} )\)

Answer 1

The Correct Option is A

Plasmid pBR322 and Its Cloning Sites 

The plasmid pBR322 is a widely used E. coli cloning vector. It contains several restriction enzyme recognition sites that can be used for inserting foreign DNA. The rop gene in pBR322 codes for proteins involved in the replication of the plasmid.

The restriction enzyme Pvu II has a unique recognition site within the rop gene of pBR322. Insertion of foreign DNA at this site would disrupt the rop gene function, potentially affecting the plasmid copy number. The other restriction enzymes listed (Pst I, EcoR I, BamH I) have cloning sites in different regions of the pBR322 plasmid, primarily within the antibiotic resistance genes (ampicillin resistance or tetracycline resistance) or outside the rop gene.

Therefore, the cloning site present in the rop site of E. coli cloning vector pBR322 is for Pvu II.