Question

While isolating DNA from bacteria the following enzyme is not used:

• A. Lysozyme
• B. Ribonuclease
• C. Deoxyribonuclease
• D. Protease

Hint:

Always ensure the absence of deoxyribonuclease in DNA extraction protocols to prevent degradation of the target DNA.

Answer 1

The Correct Option is C

During the isolation of DNA from bacteria, enzymes such as lysozyme are used to break down the cell wall, and protease is used to degrade proteins that could contaminate the DNA sample. Ribonuclease is used to remove RNA. 
However, deoxyribonuclease, which breaks down DNA, is not used as it would degrade the DNA we are trying to isolate.