Question

Which type of microscopy uses a special condenser to direct light onto the specimen from oblique angles only?

• A. Darkfield
• B. Interference contrast
• C. Phase-contrast
• D. Polarizing

Hint:

Darkfield microscopy enhances contrast in specimens that are otherwise difficult to visualize, such as living cells or thin structures like spirochetes.

Answer 1

The Correct Option is A

Darkfield microscopy employs a special condenser that directs light onto the specimen at oblique angles. This setup causes light to scatter when it hits the specimen, making bright objects appear against a dark background. It is particularly useful for observing transparent or unstained specimens like live microorganisms.