Question

Which one of the following is not a process of DNA recombinant technology?

• A. Isolation of DNA
• B. RNA interference (RNAi)
• C. Introduction of Restriction endonucleases
• D. Culturing the host cells in a medium at a large scale

Answer 1

The Correct Option is B

RNA interference (RNAi) is a biological process where RNA molecules inhibit gene expression or translation by neutralizing targeted mRNA molecules. This is not a standard part of recombinant DNA technology, which typically involves isolating DNA, using restriction enzymes, and culturing modified cells.

Answer 2

DNA recombinant technology involves various processes for manipulating DNA, such as:

• Isolation of DNA: Extracting DNA from cells is the first step in recombinant DNA technology.
• Introduction of restriction endonucleases: These enzymes are used to cut DNA at specific sequences, which is essential for cloning or inserting DNA into vectors.
• Culturing the host cells in a medium at a large scale: Once recombinant DNA is introduced into host cells, culturing them is essential to replicate and express the gene.

On the other hand, RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression by destroying specific mRNA molecules. While it is a powerful tool for regulating gene expression, it is not directly part of the processes involved in recombinant DNA technology.