Which of the following restriction enzymes produces blunt ends ?
- Sal I
- Eco RV
- Xho I
- Hind III
The Correct Option is B
Solution and Explanation
The correct answer is:
Option 2: Eco RV
Explanation:
Restriction enzymes are proteins that cut DNA at specific sequences, often creating either blunt ends or sticky ends (also known as cohesive ends). Here's a breakdown of each of the given enzymes:
Sal I: This enzyme generates sticky ends. It recognizes the sequence 5'-GTCGAC-3' and cuts between the G and T.
Eco RV: This enzyme generates blunt ends. It recognizes the sequence 5'-GATATC-3' and cuts between the A and T on both strands, leaving no overhanging bases (i.e., blunt ends).
Xho I: This enzyme generates sticky ends. It recognizes the sequence 5'-CTCGAG-3' and cuts between the C and T.
Hind III: This enzyme generates sticky ends. It recognizes the sequence 5'-AAGCTT-3' and cuts between the A and A.
Thus, Eco RV is the correct enzyme that produces blunt ends.
Top Questions on Biotechnology
- In the enzyme-linked antibody used in ELISA, the interaction between the enzyme and antibody is stabilized by
- CUET (PG) - 2025
- Bioinformatics
- Biotechnology
- Agrobacterium tumefaciens is frequently used as a vector to create transgenic plants. Under laboratory conditions Agrobacterium - mediated plant transformation does not require
- CUET (PG) - 2025
- Bioinformatics
- Biotechnology
- Ripening delayed tomatoes are produced by
- CUET (PG) - 2025
- Bioinformatics
- Biotechnology
- Monoclonal antibodies can be employed for
(A). Early detection of cancers
(B). Clear detection of pathogens
(C). Classification of blood group
Choose the correct answer from the options given below:- CUET (PG) - 2025
- Bioinformatics
- Biotechnology
- Which of the following are the properties of a good cloning vector
(A). Ideally should be less than 10 kb
(B). Isolation and purification should be easy
(C). Should contain a unique target site
(D). Able to replicate autonomously
Choose the correct answer from the options given below:- CUET (PG) - 2025
- Bioinformatics
- Biotechnology
Questions Asked in NEET exam
- A body weighs 48 N on the surface of the earth. The gravitational force experienced by the body due to the earth at a height equal to one-third the radius of the earth from its surface is:
- NEET (UG) - 2025
- Gravitational potential energy
A bob of heavy mass \(m\) is suspended by a light string of length \(l\). The bob is given a horizontal velocity \(v_0\) as shown in figure. If the string gets slack at some point P making an angle \( \theta \) from the horizontal, the ratio of the speed \(v\) of the bob at point P to its initial speed \(v_0\) is :
- NEET (UG) - 2025
- Pendulums
- An oxygen cylinder of volume 30 litre has 18.20 moles of oxygen. After some oxygen is withdrawn from the cylinder, its gauge pressure drops to 11 atmospheric pressure at temperature \(27^\circ\)C. The mass of the oxygen withdrawn from the cylinder is nearly equal to: [Given, \(R = \frac{100}{12} \text{ J mol}^{-1} \text{K}^{-1}\), and molecular mass of \(O_2 = 32 \text{ g/mol}\), 1 atm pressure = \(1.01 \times 10^5 \text{ N/m}^2\)]
- NEET (UG) - 2025
- Ideal-gas equation and absolute temperature
- A uniform rod of mass 20 kg and length 5 m leans against a smooth vertical wall making an angle of \(60^\circ\) with it. The other end rests on a rough horizontal floor. The friction force that the floor exerts on the rod is (take \(g = 10\) m/s\(^2\)):
- NEET (UG) - 2025
- Common forces in mechanics
- A wire of resistance \(R\) is cut into 8 equal pieces. From these pieces, two equivalent resistances are made by adding four of these together in parallel. Then these two are added in series. The net effective resistance of the combination is:
- NEET (UG) - 2025
- Resistance
Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.