Which of the following restriction enzymes produces blunt ends ?
- Sal I
- Eco RV
- Xho I
- Hind III
The Correct Option is B
Solution and Explanation
The correct answer is:
Option 2: Eco RV
Explanation:
Restriction enzymes are proteins that cut DNA at specific sequences, often creating either blunt ends or sticky ends (also known as cohesive ends). Here's a breakdown of each of the given enzymes:
Sal I: This enzyme generates sticky ends. It recognizes the sequence 5'-GTCGAC-3' and cuts between the G and T.
Eco RV: This enzyme generates blunt ends. It recognizes the sequence 5'-GATATC-3' and cuts between the A and T on both strands, leaving no overhanging bases (i.e., blunt ends).
Xho I: This enzyme generates sticky ends. It recognizes the sequence 5'-CTCGAG-3' and cuts between the C and T.
Hind III: This enzyme generates sticky ends. It recognizes the sequence 5'-AAGCTT-3' and cuts between the A and A.
Thus, Eco RV is the correct enzyme that produces blunt ends.
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.