Question:

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

Updated On: Nov 13, 2025

Detection of gene mutation
Molecular diagnosis
Gene amplification
Purification of isolated protein

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The Correct Option is D

Solution and Explanation

The Polymerase Chain Reaction (PCR) is a technique used in molecular biology to amplify DNA sequences. It is a fundamental method used in various applications due to its ability to amplify a small amount of DNA across several orders of magnitude, generating millions or billions of copies of a particular DNA sequence.

Let's analyze each option to see why it is or is not an application of PCR:

Detection of gene mutation: PCR can be used to amplify DNA segments, which are then analyzed for the presence of mutations. This makes the technique highly useful for genetic testing and research, identifying specific mutations linked to diseases or conditions.
Molecular diagnosis: PCR is extensively used in molecular diagnostics, including identifying pathogens by amplifying their genetic material. It aids in diagnosing infectious diseases, genetic disorders, and even in forensic investigations.
Gene amplification: The primary purpose of PCR itself is gene amplification. It is used to create multiple copies of a segment of DNA, allowing detailed study and manipulation in various research and clinical applications.
Purification of isolated protein: This step is not an application of PCR. PCR is involved in amplifying DNA, not proteins. Protein purification requires different biochemical techniques such as chromatography, electrophoresis, or centrifugation.

Conclusion: Based on the explanation above, the correct answer is Purification of isolated protein as it does not involve the application of PCR.

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