Which of the following is not a cloning vector?
- YAC
- pBR322
- Probe
- BAC
The Correct Option is C
Approach Solution - 1
A cloning vector is a small piece of DNA, taken from a virus, plasmid, or the cell of a higher organism, into which a foreign DNA segment can be inserted for cloning purposes. The options provided are:
- YAC (Yeast Artificial Chromosome): A cloning vector used to clone large fragments of DNA.
- pBR322: A commonly used plasmid vector in recombinant DNA experiments.
- Probe: A single-stranded DNA or RNA sequence used to search for its complementary sequence in a sample; not a cloning vector.
- BAC (Bacterial Artificial Chromosome): A vector used for cloning large DNA fragments in bacterial cells.
The Probe is not a cloning vector because it serves a different purpose, primarily used for hybridization experiments to detect the presence of complementary nucleic acid sequences.
Approach Solution -2
A probe is not a cloning vector. It is a short, labeled DNA or RNA molecule used to detect the presence of a complementary sequence in a sample, typically for molecular biology techniques like hybridization. Cloning vectors, on the other hand, are used to carry and replicate foreign DNA fragments in host organisms for various purposes in genetic engineering and molecular biology.
YAC (Yeast Artificial Chromosome), pBR322, and BAC (Bacterial Artificial Chromosome) are examples of cloning vectors.
so, the correct option is (C): Probe
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