In-situ bioremediation involves treating the contamination at its source without removing the contaminated materials. Out of the given options:
Pumping contaminated groundwater to the surface for treatment: This is not in-situ as it involves extraction of water.
Excavating contaminated soil and treating it in a bioreactor: This involves moving the soil, thus not in-situ.
Injecting microorganisms directly into the contaminated site: This is an example of in-situ bioremediation because it treats the contaminants at their site without moving them.
Spraying chemical agents onto polluted surfaces: This method is ex-situ when the chemical application changes the site boundaries for contaminant treatment.
Therefore, the correct answer is: Injecting microorganisms directly into the contaminated site.