Which of the following cut the DNA from specific places
- Restriction endonuclease (EcoRI)
- Ligase
- Exonuclease
- Alkaline phosphate
The Correct Option is A
Solution and Explanation
Top Questions on Biotechnology
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer)
- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
- Which of the following condition(s) lead(s) to reduced affinity of O2 for human hemoglobin?
- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
Which of the following is/are CORRECT for the two molecules shown?
- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
- Match the pair of organisms in Group I with their community interaction pattern in Group II. \[ \begin{array}{c|c} \text{Group I} & \text{Group II} \\ \hline P:\; \text{Cattle egrets – African buffalo} & 3:\; \text{Commensalism} \\ Q:\; \text{Lynx – Fox} & 1:\; \text{Competition} \\ R:\; \text{Acacia tree – Pugnacious ants} & 4:\; \text{Mutualism} \\ S:\; \text{Leopard – Antelope} & 2:\; \text{Predation} \\ \end{array} \]
- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
- In a reaction \( A + B \to C \), the initial rate of formation of C at 25°C was measured for different initial concentrations of A and B as given. The overall order of the reaction with respect to both A and B is ................. (answer in integer)
% Requires: \usepackage{graphicx} \begin{table}[h] \centering \begin{tabular}{|c|c|c|c|} \hline Experiment & Initial [A] & Initial [B] & Initial rate of formation of C
& (mol L\textsuperscript{-1}) & (mol L\textsuperscript{-1}) & (mol L\textsuperscript{-1} s\textsuperscript{-1})
\hline 1 & 0.4 & 0.3 & 0.078
2 & 0.8 & 0.3 & 0.312
3 & 0.4 & 0.6 & 0.156
4 & 0.8 & 0.6 & 0.624
\hline \end{tabular} \caption{Experimental data showing initial concentrations and rates of formation.} \label{tab:experimental_data} \end{table} \flushleft \newpage- IIT JAM BT - 2025
- Biotechnology
- Biotechnology
Questions Asked in NEET exam
The current passing through the battery in the given circuit, is:
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A bob of heavy mass \(m\) is suspended by a light string of length \(l\). The bob is given a horizontal velocity \(v_0\) as shown in figure. If the string gets slack at some point P making an angle \( \theta \) from the horizontal, the ratio of the speed \(v\) of the bob at point P to its initial speed \(v_0\) is :
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- Two cities X and Y are connected by a regular bus service with a bus leaving in either direction every T min. A girl is driving scooty with a speed of 60 km/h in the direction X to Y. She notices that a bus goes past her every 30 minutes in the direction of her motion, and every 10 minutes in the opposite direction. Choose the correct option for the period T of the bus service and the speed (assumed constant) of the buses.
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- The intensity of transmitted light when a polaroid sheet, placed between two crossed polaroids at \(22.5^\circ\) from the polarization axis of one of the polaroids (\(I_0\) is the intensity of polarised light after passing through the first polaroid):
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A full wave rectifier circuit with diodes (\(D_1\)) and (\(D_2\)) is shown in the figure. If input supply voltage \(V_{in} = 220 \sin(100 \pi t)\) volt, then at \(t = 15\) msec:
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Concepts Used:
Discovery of DNA
DNA - Discovery
Many individuals believe that American biologist - "James Watson" and English physicist - “Francis Crick” discovered DNA in the 1950s. Actually, this is not the case. Preferably, DNA was first recognized in the late 1860s by Swiss chemist - “Friedrich Miescher”.
Read More: Structure of DNA
What actually DNA is?
DNA is self-replicating material that exists in every living organism. In easiest terms, it is a carrier of all genetic information. It contains the instructions required for organisms to develop, grow, survive, and reproduce. It’s one of the longest molecules that contain our genetic “code,” or recipe. This recipe is the initial point for our development, but DNA’s interaction with outside impacts such as our lifestyle, environment, and nutrition ultimately forms human beings.
DNA is a composition of molecules known as nucleotides. Each nucleotide comprises a sugar and phosphate group, also nitrogen bases. These nitrogen bases are further tumbledown into four types, including:
- Adenine (A)
- Cytosine (C)
- Guanine (G)
- Thymine (T)