In the context of controlling bollworm pests, genetically engineered plants incorporate specific genes to produce toxins that target these pests. The CryIAc and CryIIAb genes are part of the Cry (crystal) protein family originating from Bacillus thuringiensis (Bt), a bacterium known for naturally producing insecticidal proteins.
These Cry genes are introduced into crop plants, enabling them to produce these proteins in their tissues, which are toxic to specific insects like bollworms but generally harmless to humans and other non-target organisms. When bollworms consume parts of the Bt plant, the Cry proteins bind to receptors in the gut of the insects, which leads to cell lysis and eventually the death of the insect, thus effectively controlling their population.
Other options such as RNAi (RNA interference) and dsRNA (double-stranded RNA) represent different gene-silencing technologies and mechanisms and are not directly used for controlling bollworms in this context. Therefore, the use of the CryIAc and CryIIAb genes in genetically modified crops is the correct answer for controlling the growth of bollworms.
The CryIAc and CryIIAb genes are part of the Bacillus thuringiensis (Bt) technology, which is used to control the growth of bollworms and other pests. These genes produce Cry proteins that are toxic to certain insects, including the bollworm, when they ingest the protein. These proteins disrupt the gut cells of the pest, leading to its death.
Thus, CryIAc and CryIIAb genes effectively control the growth of bollworms.
Answer the following questions:
(a) [(i)] Explain how some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans but do not kill the Bacillus.
[(ii)] How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists?
(b) [(i)] Explain how the amplification of gene of interest is done using PCR.
[(ii)] State two applications of the desired amplified fragment of DNA.
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a) What is the technical term used for Step 4 in the above process?
(b) Which of the given two combinations of restriction enzyme should be used in Step 1? Justify your answer.
(i) EcoR I to cut the plasmid and Hind III to cut the alien DNA.
(ii) EcoR I to cut both the plasmid and alien DNA.