Question

Which of the following are the types of column chromatography?
A. Paper chromatography
B. Affinity chromatography
C. Size exclusion chromatography
D. Ion-exchange chromatography
Choose the correct answer from the options given below:

• A. A, B and D only
• B. A, B and C only
• C. A, B, C and D
• D. B, C and D only

Hint:

To distinguish chromatography types, think about the physical setup. If the stationary phase is inside a tube or column, it's column chromatography. If it's on a flat sheet (like paper or a thin layer on glass), it's planar chromatography.

Answer 1

The Correct Option is D

Step 1: Understanding the Concept:
Chromatography is a technique to separate mixtures. It is classified based on the physical setup of the stationary phase.


Column Chromatography: The stationary phase is packed inside a column.

Planar Chromatography: The stationary phase is on a flat surface.

Step 2: Detailed Explanation:
Let's classify the given techniques:


A. Paper chromatography: The stationary phase is a sheet of paper. This is planar chromatography.

B. Affinity chromatography: This technique uses a stationary phase packed in a column. This is column chromatography.

C. Size exclusion chromatography: This method uses a column packed with porous beads. This is column chromatography.

D. Ion-exchange chromatography: This technique uses a column packed with a charged resin. This is column chromatography.

Step 3: Final Answer:
Therefore, B, C, and D are types of column chromatography, while A is not. The correct option is (D).