Question

What type of culture media is used for Ligionella?

• A. (BCYE) agar media
• B. MacConkey agar
• C. Baird–Parker agar
• D. Sabouraud’s agar

Answer 1

The Correct Option is A

Legionella bacteria are typically cultured using a specific type of culture media known as Buffered Charcoal Yeast Extract (BCYE) agar media. This medium is specially formulated to support the growth of Legionella species, which are not easily cultured on general-purpose media. BCYE agar contains nutrients that are essential for Legionella, such as charcoal which aids in removing toxic molecules and yeast extract which provides organic nutrients.

Here's a brief comparison of the options:

Culture Media	Purpose
(BCYE) agar media	Specifically designed for culturing Legionella species.
MacConkey agar	Used for selective isolation and differentiation of Gram-negative bacteria.
Baird–Parker agar	Used for the isolation of Staphylococcus aureus.
Sabouraud’s agar	Designed for culturing fungi, such as yeasts and molds.

Among the given options, only (BCYE) agar media is suitable for cultivating Legionella. This makes it the correct answer.