Question

What does the sample of given base sequence represent? 5' - GAATTC - 3' 3' - CTTAAG - 5'

• A. Palindromic sequence
• B. Initiator codon at 5' end
• C. Deletion mutation
• D. Completion of replication

Answer 1

The Correct Option is A

A palindromic sequence is a DNA sequence that reads the same on both strands when read in the 5' to 3' direction. In this case, the sequence "GAATTC" on one strand is complementary to the sequence "CTTAAG" on the opposite strand.

The recognition of palindromic sequences by specific restriction enzymes is important in molecular biology. Many restriction enzymes recognize and cleave DNA at specific palindromic sequences. In this example, the palindromic sequence "GAATTC" is recognized by the restriction enzyme EcoRI, which cleaves the DNA at that specific site.

Therefore, the given base sequence represents a palindromic sequence, where the sequence on one strand is the reverse complement of the sequence on the other strand.

Answer 2

The given base sequence:

5' - GAATTC - 3'
3' - CTTAAG - 5'

represents the recognition site for the restriction enzyme EcoRI.

Explanation:

EcoRI is a restriction endonuclease that recognizes the palindromic sequence 5'-GAATTC-3' and cuts between G and A, producing sticky ends.

This enzyme is widely used in genetic engineering and recombinant DNA technology for cutting and inserting DNA fragments.

Thus, the given sequence represents a restriction site for EcoRI.