Activated sludge is a flocculent mass of microorganisms, primarily bacteria, along with fungi, protozoa, and other aerobic microbes, that develops in the aeration tank of a Sewage Treatment Plant (STP) during the biological treatment of wastewater. This sludge is called ”activated” because the microbial community within it is highly active in consuming and oxidizing the organic pollutants present in the sewage. The process involves aerating the wastewater in the presence of this microbial floc. The microorganisms utilize the organic matter as their food source, metabolizing it and converting it into microbial biomass (more sludge), carbon dioxide, and water. The flocs of activated sludge have a spongy structure that allows for efficient adsorption and degradation of organic pollutants. After the aeration tank, the activated sludge is allowed to settle in a settling tank (secondary clarifier). Most of this settled sludge is then recycled back into the aeration tank to serve as an inoculum, ensuring a continuous and efficient biological treatment process.