In eukaryotes, tRNA molecules are synthesized by RNA polymerase III, which is specialized for transcribing small RNA molecules, including 5S rRNA and tRNAs. RNA polymerase I transcribes most rRNA (except 5S rRNA), and RNA polymerase II synthesizes mRNA and some snRNAs. Ribosomes, on the other hand, are involved in translation and not RNA synthesis.
List I | List II |
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(A) Radiation hybrid mapping | (I) DNA can be cut into large fragments and circularized for use in chromosome walking |
(B) Sequence tagged site (STS) mapping | (II) Useful for cloning of overlapping DNA fragments (restricted to about 200 kb) |
(C) Chromosome jumping | (III) Fragment genome into large pieces and locate markers and genes |
(D) Chromosome walking | (IV) Applicable to any part of DNA sequence if some sequence information is available |